AustralieFrV1, Main, Exploration, bibRecord, 00DF92

Labelling of living mammalian spermatozoa with the fluorescent thiol alkylating agent, monobromobimane (MB): Immobilization upon exposure to ultraviolet light and analysis of acrosomal status

Identifieur interne : 00DF92 ( Main/Exploration ); précédent : 00DF91; suivant : 00DF93

Labelling of living mammalian spermatozoa with the fluorescent thiol alkylating agent, monobromobimane (MB): Immobilization upon exposure to ultraviolet light and analysis of acrosomal status

Auteurs : James M. Cummins [Australie] ; Alan D. Fleming [États-Unis] ; Nicole Crozet [France] ; Thomas J. Kuehl [États-Unis] ; Nechama S. Kosower [Israël] ; Ryuzo Yanagimachi [États-Unis]

Source :

Journal of Experimental Zoology [ 0022-104X ] ; 1986-03.

RBID : ISTEX:9E8B2564A72BD47C96A20A7DC43D0EAFF641CC8B

Descripteurs français

Wicri :
- topic : étiquetage, Optique.

English descriptors

Abstract

Living spermatozoa of seven mammalian species were treated with the thiol‐alkylating fluorescent labelling compound, monobromobimane (MBBR). MB‐labelling alone had no effect on sperm motility, nor on the time course or ability of golden hamster spermatozoa to undergo the acrosome reaction when capacitated in vitro. Exposure of MB‐labelled spermatozoa to ultraviolet (UV) light and excitation of the MB fluorochrome resulted in virtually immediate immobilization of the spermatozoa without affecting acrosomal status. UV exposure of unlabelled spermatozoa for up to 30 sec had no effect upon motility. Immobilization of MB‐labelled spermatozoa depended on the midpiece being irradiated, as irradiation of the head alone, or of the more distal parts of the principal piece, had little or no effect upon motility. Labelling with MB followed by immobilization of individually selected spermatozoa was most useful for detailing the course and site of occurrence of the acrosome reaction during penetration of the cumulus oophorus by golden hamster spermatozoa in vitro. In these often hyperactivated spermatozoa, precise determination of the acrosomal status could not often otherwise be made due to the difficulty in visualizing the acrosomal region of a vigorously thrashing, hyper‐activated spermatozoon. This technique should prove valuable in a variety of studies on sperm motility, capacitation and fertilization, and could also be extended to other cell systems.

Url:

https://api.istex.fr/document/9E8B2564A72BD47C96A20A7DC43D0EAFF641CC8B/fulltext/pdf

DOI: 10.1002/jez.1402370310

Affiliations:

Australie, France, Israël, États-Unis
Hawaï, Texas

Links toward previous steps (curation, corpus...)

to stream Istex, to step Corpus: 001D97
to stream Istex, to step Curation: 001D97
to stream Istex, to step Checkpoint: 002E23
to stream Main, to step Merge: 00F230
to stream Main, to step Curation: 00DF92

Le document en format XML

<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Labelling of living mammalian spermatozoa with the fluorescent thiol alkylating agent, monobromobimane (MB): Immobilization upon exposure to ultraviolet light and analysis of acrosomal status</title>
<author><name sortKey="Cummins, James M" sort="Cummins, James M" uniqKey="Cummins J" first="James M." last="Cummins">James M. Cummins</name>
</author>
<author><name sortKey="Fleming, Alan D" sort="Fleming, Alan D" uniqKey="Fleming A" first="Alan D." last="Fleming">Alan D. Fleming</name>
</author>
<author><name sortKey="Crozet, Nicole" sort="Crozet, Nicole" uniqKey="Crozet N" first="Nicole" last="Crozet">Nicole Crozet</name>
</author>
<author><name sortKey="Kuehl, Thomas J" sort="Kuehl, Thomas J" uniqKey="Kuehl T" first="Thomas J." last="Kuehl">Thomas J. Kuehl</name>
</author>
<author><name sortKey="Kosower, Nechama S" sort="Kosower, Nechama S" uniqKey="Kosower N" first="Nechama S." last="Kosower">Nechama S. Kosower</name>
</author>
<author><name sortKey="Yanagimachi, Ryuzo" sort="Yanagimachi, Ryuzo" uniqKey="Yanagimachi R" first="Ryuzo" last="Yanagimachi">Ryuzo Yanagimachi</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:9E8B2564A72BD47C96A20A7DC43D0EAFF641CC8B</idno>
<date when="1986" year="1986">1986</date>
<idno type="doi">10.1002/jez.1402370310</idno>
<idno type="url">https://api.istex.fr/document/9E8B2564A72BD47C96A20A7DC43D0EAFF641CC8B/fulltext/pdf</idno>
<idno type="wicri:Area/Istex/Corpus">001D97</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001D97</idno>
<idno type="wicri:Area/Istex/Curation">001D97</idno>
<idno type="wicri:Area/Istex/Checkpoint">002E23</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">002E23</idno>
<idno type="wicri:doubleKey">0022-104X:1986:Cummins J:labelling:of:living</idno>
<idno type="wicri:Area/Main/Merge">00F230</idno>
<idno type="wicri:Area/Main/Curation">00DF92</idno>
<idno type="wicri:Area/Main/Exploration">00DF92</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Labelling of living mammalian spermatozoa with the fluorescent thiol alkylating agent, monobromobimane (MB): Immobilization upon exposure to ultraviolet light and analysis of acrosomal status</title>
<author><name sortKey="Cummins, James M" sort="Cummins, James M" uniqKey="Cummins J" first="James M." last="Cummins">James M. Cummins</name>
<affiliation wicri:level="1"><country xml:lang="fr">Australie</country>
<wicri:regionArea>Department of Veterinary Anatomy, University of Queensland, St. Lucia</wicri:regionArea>
<wicri:noRegion>St. Lucia</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Fleming, Alan D" sort="Fleming, Alan D" uniqKey="Fleming A" first="Alan D." last="Fleming">Alan D. Fleming</name>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Texas</region>
</placeName>
<wicri:cityArea>Perinatal Biology Program, Southwest Foundation for Biomedical Research, San Antonio</wicri:cityArea>
</affiliation>
<affiliation></affiliation>
</author>
<author><name sortKey="Crozet, Nicole" sort="Crozet, Nicole" uniqKey="Crozet N" first="Nicole" last="Crozet">Nicole Crozet</name>
<affiliation wicri:level="1"><country xml:lang="fr">France</country>
<wicri:regionArea>INRA, Station Physiologie Animale, Jouy en Josas</wicri:regionArea>
<wicri:noRegion>Jouy en Josas</wicri:noRegion>
<wicri:noRegion>Jouy en Josas</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kuehl, Thomas J" sort="Kuehl, Thomas J" uniqKey="Kuehl T" first="Thomas J." last="Kuehl">Thomas J. Kuehl</name>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Texas</region>
</placeName>
<wicri:cityArea>Perinatal Biology Program, Southwest Foundation for Biomedical Research, San Antonio</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Kosower, Nechama S" sort="Kosower, Nechama S" uniqKey="Kosower N" first="Nechama S." last="Kosower">Nechama S. Kosower</name>
<affiliation wicri:level="1"><country xml:lang="fr">Israël</country>
<wicri:regionArea>Department of Human Genetics, Tel‐Aviv University School of Medicine, Tel‐Aviv</wicri:regionArea>
<wicri:noRegion>Tel‐Aviv</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Yanagimachi, Ryuzo" sort="Yanagimachi, Ryuzo" uniqKey="Yanagimachi R" first="Ryuzo" last="Yanagimachi">Ryuzo Yanagimachi</name>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Hawaï</region>
</placeName>
<wicri:cityArea>Department of Anatomy and Reproductive Biology, University of Hawaii School of Medicine, Honolulu</wicri:cityArea>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j" type="main">Journal of Experimental Zoology</title>
<title level="j" type="alt">JOURNAL OF EXPERIMENTAL ZOOLOGY</title>
<idno type="ISSN">0022-104X</idno>
<idno type="eISSN">1097-010X</idno>
<imprint><biblScope unit="vol">237</biblScope>
<biblScope unit="issue">3</biblScope>
<biblScope unit="page" from="375">375</biblScope>
<biblScope unit="page" to="382">382</biblScope>
<biblScope unit="page-count">8</biblScope>
<publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher>
<pubPlace>New York</pubPlace>
<date type="published" when="1986-03">1986-03</date>
</imprint>
<idno type="ISSN">0022-104X</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0022-104X</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acrosomal</term>
<term>Acrosomal status</term>
<term>Acrosome</term>
<term>Acrosome reaction</term>
<term>Acrosome reactions</term>
<term>Baboon</term>
<term>Baboon spermatozoa</term>
<term>Capacitation</term>
<term>Chimpanzee</term>
<term>Chimpanzee spermatozoa</term>
<term>Cumulus</term>
<term>Epifluorescence</term>
<term>Epifluorescence optics</term>
<term>Fluorescent labelling</term>
<term>Golden hamster</term>
<term>Golden hamster spermatozoa</term>
<term>Golden hamster spermatozoon</term>
<term>Hamster</term>
<term>Hamster spermatozoa</term>
<term>Head region</term>
<term>Human spermatozoa</term>
<term>Immediate immobilization</term>
<term>Immobilization</term>
<term>Kosower</term>
<term>Labelling</term>
<term>Midpiece</term>
<term>Motile</term>
<term>Motile spermatozoa</term>
<term>Motility</term>
<term>Optics</term>
<term>Principal piece</term>
<term>Sperm</term>
<term>Sperm motility</term>
<term>Sperm suspension</term>
<term>Spermatozoon</term>
<term>Unlabelled</term>
<term>Unlabelled spermatozoa</term>
<term>Yanagimachi</term>
</keywords>
<keywords scheme="Teeft" xml:lang="en"><term>Acrosomal</term>
<term>Acrosomal status</term>
<term>Acrosome</term>
<term>Acrosome reaction</term>
<term>Acrosome reactions</term>
<term>Baboon</term>
<term>Baboon spermatozoa</term>
<term>Capacitation</term>
<term>Chimpanzee</term>
<term>Chimpanzee spermatozoa</term>
<term>Cumulus</term>
<term>Epifluorescence</term>
<term>Epifluorescence optics</term>
<term>Fluorescent labelling</term>
<term>Golden hamster</term>
<term>Golden hamster spermatozoa</term>
<term>Golden hamster spermatozoon</term>
<term>Hamster</term>
<term>Hamster spermatozoa</term>
<term>Head region</term>
<term>Human spermatozoa</term>
<term>Immediate immobilization</term>
<term>Immobilization</term>
<term>Kosower</term>
<term>Labelling</term>
<term>Midpiece</term>
<term>Motile</term>
<term>Motile spermatozoa</term>
<term>Motility</term>
<term>Optics</term>
<term>Principal piece</term>
<term>Sperm</term>
<term>Sperm motility</term>
<term>Sperm suspension</term>
<term>Spermatozoon</term>
<term>Unlabelled</term>
<term>Unlabelled spermatozoa</term>
<term>Yanagimachi</term>
</keywords>
<keywords scheme="Wicri" type="topic" xml:lang="fr"><term>étiquetage</term>
<term>Optique</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Living spermatozoa of seven mammalian species were treated with the thiol‐alkylating fluorescent labelling compound, monobromobimane (MBBR). MB‐labelling alone had no effect on sperm motility, nor on the time course or ability of golden hamster spermatozoa to undergo the acrosome reaction when capacitated in vitro. Exposure of MB‐labelled spermatozoa to ultraviolet (UV) light and excitation of the MB fluorochrome resulted in virtually immediate immobilization of the spermatozoa without affecting acrosomal status. UV exposure of unlabelled spermatozoa for up to 30 sec had no effect upon motility. Immobilization of MB‐labelled spermatozoa depended on the midpiece being irradiated, as irradiation of the head alone, or of the more distal parts of the principal piece, had little or no effect upon motility. Labelling with MB followed by immobilization of individually selected spermatozoa was most useful for detailing the course and site of occurrence of the acrosome reaction during penetration of the cumulus oophorus by golden hamster spermatozoa in vitro. In these often hyperactivated spermatozoa, precise determination of the acrosomal status could not often otherwise be made due to the difficulty in visualizing the acrosomal region of a vigorously thrashing, hyper‐activated spermatozoon. This technique should prove valuable in a variety of studies on sperm motility, capacitation and fertilization, and could also be extended to other cell systems.</div>
</front>
</TEI>
<affiliations><list><country><li>Australie</li>
<li>France</li>
<li>Israël</li>
<li>États-Unis</li>
</country>
<region><li>Hawaï</li>
<li>Texas</li>
</region>
</list>
<tree><country name="Australie"><noRegion><name sortKey="Cummins, James M" sort="Cummins, James M" uniqKey="Cummins J" first="James M." last="Cummins">James M. Cummins</name>
</noRegion>
</country>
<country name="États-Unis"><region name="Texas"><name sortKey="Fleming, Alan D" sort="Fleming, Alan D" uniqKey="Fleming A" first="Alan D." last="Fleming">Alan D. Fleming</name>
</region>
<name sortKey="Kuehl, Thomas J" sort="Kuehl, Thomas J" uniqKey="Kuehl T" first="Thomas J." last="Kuehl">Thomas J. Kuehl</name>
<name sortKey="Yanagimachi, Ryuzo" sort="Yanagimachi, Ryuzo" uniqKey="Yanagimachi R" first="Ryuzo" last="Yanagimachi">Ryuzo Yanagimachi</name>
</country>
<country name="France"><noRegion><name sortKey="Crozet, Nicole" sort="Crozet, Nicole" uniqKey="Crozet N" first="Nicole" last="Crozet">Nicole Crozet</name>
</noRegion>
</country>
<country name="Israël"><noRegion><name sortKey="Kosower, Nechama S" sort="Kosower, Nechama S" uniqKey="Kosower N" first="Nechama S." last="Kosower">Nechama S. Kosower</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Asie/explor/AustralieFrV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 00DF92 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 00DF92 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Asie
   |area=    AustralieFrV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     ISTEX:9E8B2564A72BD47C96A20A7DC43D0EAFF641CC8B
   |texte=   Labelling of living mammalian spermatozoa with the fluorescent thiol alkylating agent, monobromobimane (MB): Immobilization upon exposure to ultraviolet light and analysis of acrosomal status
}}

This area was generated with Dilib version V0.6.33.
Data generation: Tue Dec 5 10:43:12 2017. Site generation: Tue Mar 5 14:07:20 2024

	Serveur d'exploration sur les relations entre la France et l'Australie
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration sur les relations entre la France et l'Australie

Labelling of living mammalian spermatozoa with the fluorescent thiol alkylating agent, monobromobimane (MB): Immobilization upon exposure to ultraviolet light and analysis of acrosomal status

Labelling of living mammalian spermatozoa with the fluorescent thiol alkylating agent, monobromobimane (MB): Immobilization upon exposure to ultraviolet light and analysis of acrosomal status

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri